Title: ``Use of macroarray technology for the identification of plant genes that are differentially expressed during the initial stages of Agrobacterium-mediated transformation''
Speaker: Dr. Veena, (Gelvin Lab) Department of Biological Sciences, Purdue University
Place: UNIV 101; Tuesday, 4:30pm


We have used a combination of suppressive subtractive hybridization and macroarray technologies to identify plant genes that are differentially regulated during the initial stages of Agrobacterium-mediated transformation. Agrobacterium-mediated transformation is the most popular method currently in use for generating transgenic plants. However, very little is known about plant factors involved in this process. Identification and molecular characterization of differentially expressed genes during early stages of Agrobacterium-mediated transformation may provide a better understanding of the plant response to Agrobacterium infection. Such information may help to develop methods to enhance the frequency of Agrobacterium-mediated transformation of recalcitrant plant species and varieties. Conversely, these results may also be used to prevent crown gall disease in various plants susceptible to Agrobacterium infection. This talk will focus on the experimental details and methodologies of macroarray technology used in the present study. Note: Statistical analysis of these data will be presented in a upcoming seminar on February 19, 2002.