Tuesday, September 1, 2009
04:30 PM in HORT 117
Professor Rick Westerman
Bioinformatics Specialist, Genomics Facility, Purdue University

Second generation sequencing: An overview plus some case studies

Abstract

The advent of second generation (2nd gen) sequencing during the last couple of years has been rapidly displacing the monopoly enjoyed by Sanger sequencing. The current 2nd gen systems generate much more data at a lower cost than previous sequencers. This massive amount of data is presenting new challenges in data analysis as well as spurring on innovative methods in genomic research. The Genomics Facility at Purdue has two of the three common 2nd gen "sequencing by synthesis" systems the Roche FLX/454 system and the ABI SOLiD system (we do not have the Illumina/Solexa system.) My talk will present an overview of 2nd gen sequencing technology, cover some of the innovative algorithms that are being produced worldwide, and then delve into some of the real life problems and solutions that we have encountered while using our 2nd gen systems.

Associated Reading:

Next-generation DNA sequencing methods. Elaine R. Mardis. Annual Review of Genomics and Human Genetics. 2008. 9:387-402.

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